ROLE OF REGULATORY FACTOR X1 IN HUMAN GBM
Led by Zhiyi Zuo at University of Virginia
Glioblastoma multiforme (GBM) is the most common and most deadly malignant primary brain tumor in humans. Our preliminary results showed that a protein called regulatory factor X1 (RFX1) inhibited the survival, proliferation and trans-well invasion of GBM cells as well as the in vivo growth of human glioblastoma xenografts. We also showed that RFX1 reduced the level of a protein called clusters of differentiation 44 (CD44). CD44 plays an important role in regulating tumor cell growth, survival and invasion. In this project, we will determine whether: 1) RFX1 directly down-regulates CD44 in glioblastoma, and 2) RFX1 is down-regulated in human glioblastoma tissues and its expression inversely correlates with CD44 expression. We will use various types of GBM cells. Their levels of RFX1 will be increased by a biochemical method. The levels of CD44 in these cells and the cells that have normal levels of RFX1 will be compared. Advanced biochemical techniques will be used to determine whether RFX1 binds to the CD44 gene and affects the activity of the gene. Human normal and GBM brain tissues will be used to measure the levels of RFX1 and CD44. These studies will not only advance our knowledge of glioblastoma biology but also identify potential targets for improving the outcome of this deadly disease.
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